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Sc 955 Rrid Ab 2166917, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
P300 Rabbit Monoclonal Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a300 358a rrid ab 185565  (Bethyl)
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
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Lc IRF11 recruits <t>p300</t> and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.
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Image Search Results


Journal: Cell reports

Article Title: Increased nuclear factor I-mediated chromatin access drives transition to androgen receptor splice variant dependence in prostate cancer

doi: 10.1016/j.celrep.2024.115089

Figure Lengend Snippet:

Article Snippet: anti-p300 , Cell Signaling Technologies , cat57625S; RRID:AB_3068009.

Techniques: Recombinant, Virus, Control, Multiplex Assay, Cell Culture, Purification, Bicinchoninic Acid Protein Assay, Fractionation, CyQUANT Assay, Proliferation Assay, Gene Expression, Expressing, Sequencing, Software, Combined Bisulfite Restriction Analysis Assay

Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.

Journal: The Journal of Biological Chemistry

Article Title: P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11

doi: 10.1016/j.jbc.2025.108193

Figure Lengend Snippet: Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. A and B , Lc IRF11 recruits p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with Lc IRF11 expression plasmid (10 μg) for 36 h. ChIP analysis was performed to determine the levels of p300 or Pol II using their respective Abs. C and D , knockdown of Lc IRF11 hindered the r Lc IFNi-induced recruitment of p300 and Pol II to the Lc MDA5 promoter region. LYCK cells (1 × 10 7 cells) were transfected with siRNAs (100 nM) targeting Lc IRF11 for 24 h, followed by treatment with r Lc IFNi (250 ng/ml) for an additional 12 h. ChIP analysis determined the levels of p300 or Pol II using their respective Abs. Normal mouse/rabbit IgG (3 μg) served as the negative control. The ChIP-enriched DNA underwent subsequent qPCR analysis. The presented data represent the mean ± SD from three biological replicates (∗ p < 0.05, two-tailed unpaired Student's t test). E , protein pull-down assays confirmed the interaction of Lc IRF11 with both p300 and Pol II. LYCK cell lysates were incubated with r Lc IRF11 protein or control protein (cell lysates from pcDNA3.1 vector-transfected cells) at 4 °C for 2 h, and the protein interactions were detected using specific Abs.

Article Snippet: The Abs utilized in this study were as follows: Flag mouse monoclonal Ab (MBL, cat#M185-3L, 1:10,000 for Western blot); STAT1 rabbit polyclonal Ab (Cell Signaling Technology, cat#9172S, 1:1000 for Western blot); H3K4me3 rabbit polyclonal Ab (Thermo Fisher Scientific, cat#711958, 3 μg for ChIP); H3K27ac rabbit polyclonal Ab (Thermo Fisher Scientific, cat#PA5-96618, 3 μg for ChIP); p300 rabbit monoclonal Ab (Cell Signaling Technology, cat#57625, 1:100 for ChIP, 1:1000 for Western blot); POLR1A mouse monoclonal Ab (Pol II subunit RPB1, 8WG16, Thermo Fisher Scientific, cat#MA1-26249, 3 μg for ChIP, 1:1000 for Western blot); β-tubulin mouse monoclonal Ab (Beyotime; cat#AF2839, 1:1000 for Western blot), and HRP-conjugated goat anti mouse/rabbit secondary Abs (Proteintech, cat#SA00001-1, cat#SA00001-2, 1:10,000).

Techniques: Transfection, Expressing, Plasmid Preparation, Knockdown, Negative Control, Two Tailed Test, Incubation, Control

Model of IRF11-mediated MDA5 expression. The type I IFN ( Lc IFNi) activates JAK-STAT signaling and phosphorylates Lc IRF11. Phosphorylated Lc IRF11 then enters the nucleus and associates with the ISRE (also known as IRF binding element, IRF-E) motif of the Lc MDA5 promoter via the α3 helix and loop 1, with assistance from loop 2 and loop 3 within its DBD domain. Subsequently, IRF11 recruits p300 and Pol II to the MDA5 promoter region, resulting in the elevation of histone H3K27ac and H3K4me3 levels in the corresponding genomic region. L1, loop 1; L2, loop 2; L3, loop 3; α3, α3 helix.

Journal: The Journal of Biological Chemistry

Article Title: P300/RNA polymerase II mediates induction of the teleost viral RNA sensor MDA5 through the interferon regulatory factor IRF11

doi: 10.1016/j.jbc.2025.108193

Figure Lengend Snippet: Model of IRF11-mediated MDA5 expression. The type I IFN ( Lc IFNi) activates JAK-STAT signaling and phosphorylates Lc IRF11. Phosphorylated Lc IRF11 then enters the nucleus and associates with the ISRE (also known as IRF binding element, IRF-E) motif of the Lc MDA5 promoter via the α3 helix and loop 1, with assistance from loop 2 and loop 3 within its DBD domain. Subsequently, IRF11 recruits p300 and Pol II to the MDA5 promoter region, resulting in the elevation of histone H3K27ac and H3K4me3 levels in the corresponding genomic region. L1, loop 1; L2, loop 2; L3, loop 3; α3, α3 helix.

Article Snippet: The Abs utilized in this study were as follows: Flag mouse monoclonal Ab (MBL, cat#M185-3L, 1:10,000 for Western blot); STAT1 rabbit polyclonal Ab (Cell Signaling Technology, cat#9172S, 1:1000 for Western blot); H3K4me3 rabbit polyclonal Ab (Thermo Fisher Scientific, cat#711958, 3 μg for ChIP); H3K27ac rabbit polyclonal Ab (Thermo Fisher Scientific, cat#PA5-96618, 3 μg for ChIP); p300 rabbit monoclonal Ab (Cell Signaling Technology, cat#57625, 1:100 for ChIP, 1:1000 for Western blot); POLR1A mouse monoclonal Ab (Pol II subunit RPB1, 8WG16, Thermo Fisher Scientific, cat#MA1-26249, 3 μg for ChIP, 1:1000 for Western blot); β-tubulin mouse monoclonal Ab (Beyotime; cat#AF2839, 1:1000 for Western blot), and HRP-conjugated goat anti mouse/rabbit secondary Abs (Proteintech, cat#SA00001-1, cat#SA00001-2, 1:10,000).

Techniques: Expressing, Binding Assay